In order to explore the factors affecting the operation effect of ozone oxidation process and optimize the operation parameters, the treatment mechanism of ozone oxidation process was introduced. The effects of environmental conditions, ozone dosage and ozone contact time on the ozone oxidation efficiency were analyzed, and the optimal ozone dosage and contact time were defined based on literature. The analysis shows that the rise of temperature can increase the ozone consumption rate and reaction rate. However,it has little effect on the removal of organic matter. The optimum pH is 7. The range of optimum ozone dosage is about O3/COD=0.13~0.25 or O3/TOC=0.5~1.2. When the TOC concentration of secondary effluent is higher than 12 mg/L, the optimal ozone contact time is 4~5 min. When the TOC concentration of secondary effluent is lower than 12 mg/L, the optimal ozone contact time is 10~15 min.