Abstract: Taking bisphenol A( BHA) in simulated surface water as the target pollutant,using PDLGAas spinning materials,electrospun laccase-carrying nanofiber membranes were prepared with electro-spinning immobilized laccase. The degradation performance of BPA was investigated. The influencingfactors such as temperature and pH were analyzed. The results showed that the activity retention rate oflaccase immobilized on electrospun nanofiber membrane to free enzyme was 78.5%. The two-hour deg-radation efficiencies of low concentration( 100 μg /L) and high concentration( 100 mg /L) of BPA byimmobilized laccase reached 83.5% and 31.7%,respectively. Within the temperature range of 5 ~45 ℃,the ranges of degradation efficiency of BPA by immobilized enzyme and free enzyme were31.2% ~71.2% and 29.8% ~ 89.4%,respectively. Within the pH range of 1 ~ 9,the ranges ofdegradation efficiency of BPA by immobilized enzyme and free enzyme were 12.2% ~64.7% and 0 ~59.8%,respectively. Close-