In order to reveal the key pollution sources and main pollution areas of agricultural non-point source pollution in Heze City, the statistical data of Heze City from 2012 to 2021 was analyzed with GIS technology. The spatial distribution map of pollutants was drawn, the pollution emissions were calculated, and the temporal and spatial distribution characteristics of agricultural non-point source pollution were analyzed. The results showed that the application intensity of chemical fertilizer in Heze City decreased year by year,but it was still higher than the international early warning value of chemical fertilizer (225 kg·hm-2) . The chemical fertilizer pollution in the northwest of Heze City was more serious than the other areas. The number of poultry breeding gradually increased. The level of livestock and poultry pollution in the north and west of Heze City was medium pollution. After 2015, the use of chemical fertilizer and pesticide steadily decreased by 12.52% and 18.91%, respectively.