This study deals with laboratory-scale ozone oxidation test of simulated aniline wastewater. Relevant water quality indicators have been analyzed, including pH, TOC, COD, N-related parameters (ammonium, TKN, nitrite, nitrate), aniline, nitrobenzene and color. Results show that all indicators reveal different change trend in process of reaction. Meanwhile, preliminary investigation on its mechanism has also been conducted by referring to change of each indicator. Finally, some advice has been given to improve engineering design. In order to improve the treatment effect of aniline wastewater, the ozone oxidation static test was used to treat simulated aniline wastewater. The pollutant removal efficiency and reaction mechanism were analyzed. The results show that the removal rate of aniline increases with the increase of ozone dosage. When the ozone dosage is 116 mg/L, the removal rates of aniline, COD and TOC are 99.9%, 64.5% and 10.8%, respectively. The optimum reaction pH is 7. When the ozone dosage gradually increases to 116 mg/L, the concentration of NH+4-N increases from 7.65 mg/L to 23.33 mg/L, but the concentrations of TN, NO-2-N and NO-3-N have no significant change. Aniline oxidation is the result of direct oxidation and indirect oxidation of ozone. The maleic acid produced during the reaction process leads to the decrease of pH. Quinones and imines are the main by-products of oxidation reaction.